Re: [Histonet] Auto stainer IHC/SS

From:"Promila Rastogi"

FYI, one CAN do IHC, ISH and special stains in the same run on the i6000
Automated Stainer from BioGenex.


Promila A. Rastogi
Product Marketing Manager

-----Original Message-----
[] On Behalf Of Tom
Sent: Friday, November 18, 2005 8:34 AM
To: HISTONET (E-mail)
Subject: RE: [Histonet] Auto stainer IHC/SS

The special stains thatI have run have all been very nice except for
masson's trichrome.  We just don't like it.  It is an open system though
and I can use my own reagents and tweak it, just haven't had time.  You
cannot do IHC and special stains at the same time... they have to be
separate runs.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Hospital
Newark, Ohio  43055 

-----Original Message-----
From: Lewis, Sarah []
Sent: Wednesday, November 16, 2005 11:10 AM
To: HISTONET (E-mail)
Subject: [Histonet] Auto stainer IHC/SS

Just a quick question in regards to the Autostainer Plus from Dako. I am
currently looking to purchase an Automated Immuno Stainer. I have demoed
The i6000, Mozaic, and the Nemesis.  I will soon demo the Autostainer
Plus from Dako.  They claim to be able to do special stains and IHC. Are
any of you running specials on your Autostainer plus?  Any thoughts on
this?  Also,  If you have any experience with any of the units any
feedback would be appreciated!!!  The i6000 also claims to do specials
as well,  I only ran a few,  I never tried to run IHC and specials
together on the same run.(They have disposable pipette tips which
eliminates the cross contamination
issue.)      Any thoughts would be great!!! Thanks in advance!!!

Sarah E. Lewis
Childrens Research
Center for Gene Therapy
700 Childrens Dr Rm WA3112
Columbus OH 43205

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