RE: [Histonet] oil red o

From:"Rogerson Kemlo \(ELHT\) Pathology"

Um nope, not even near.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan
Llewellyn
Sent: 21 November 2005 17:07
To: Histonet
Subject: Re: [Histonet] oil red o

The ending "er" in English denotes a person (or thing) who/which does an

action.  To "bug" is a colloquial expression meaning to irritate, so a 
"bugger" (the letter g is doubled to preserve the short vowel sound)
would 
be someone (or something) who irritates someone else.  Is there another 
meaning?

Bryan




----- Original Message ----- 
From: "Rogerson Kemlo (ELHT) Pathology" 
To: "Kristen Broomall" ; "Sally Prouty" 
; 
Sent: Monday, November 21, 2005 8:28 AM
Subject: RE: [Histonet] oil red o


"Quite a bugger" I quote.

Do you know what one is? It doesn't seem to fit the context in which you
are using it. Are you using it as a noun or a verb?

I'm sure you aren't talking about Sally.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kristen
Broomall
Sent: Monday, November 21, 2005 4:19 PM
To: 'Sally Prouty'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] oil red o

Make sure that you are not pressing (at all!) on your coverslip, even to
get
out air bubbles. The ORO will come right out of the fat cells. Quite a
bugger.

Kristen

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sally
Prouty
Sent: Sunday, November 20, 2005 11:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] oil red o


I am trying to locate a protocol for a consistant Oil red O stain. I
don't
know if it is in coverslipping where the stain is moving off the fat
cells
or in my differentiation step. I am staining on frozen skeletal muscle
sections. Any suggestions would be helpful. Thank you,

Sally


Sally J. Prouty
HHMI/University of Iowa
Kevin Campbell Laboratory
400 EMRB
Iowa City, Iowa 52246
319-335-6944

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