RE: [Histonet] oil red o

From:"Kristen Broomall"

Make sure that you are not pressing (at all!) on your coverslip, even to get
out air bubbles. The ORO will come right out of the fat cells. Quite a


-----Original Message-----
[]On Behalf Of Sally
Sent: Sunday, November 20, 2005 11:53 PM
Subject: [Histonet] oil red o

I am trying to locate a protocol for a consistant Oil red O stain. I don't 
know if it is in coverslipping where the stain is moving off the fat cells 
or in my differentiation step. I am staining on frozen skeletal muscle 
sections. Any suggestions would be helpful. Thank you,


Sally J. Prouty
HHMI/University of Iowa
Kevin Campbell Laboratory
400 EMRB
Iowa City, Iowa 52246

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