RE: [Histonet] oil red o
Make sure that you are not pressing (at all!) on your coverslip, even to get
out air bubbles. The ORO will come right out of the fat cells. Quite a
bugger.
Kristen
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sally
Prouty
Sent: Sunday, November 20, 2005 11:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] oil red o
I am trying to locate a protocol for a consistant Oil red O stain. I don't
know if it is in coverslipping where the stain is moving off the fat cells
or in my differentiation step. I am staining on frozen skeletal muscle
sections. Any suggestions would be helpful. Thank you,
Sally
Sally J. Prouty
HHMI/University of Iowa
Kevin Campbell Laboratory
400 EMRB
Iowa City, Iowa 52246
319-335-6944
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