RE: [Histonet] Re: 70% from NBF
We also used chloroform extensively and found that for some tissues it
was greatly superior to xylene.
Some tissue such as bone can be left in chloroform for extended periods
of time without becoming too hard or brittle. We first noticed this when
some rat adrenals were left in chloroform for 2 weeks (in error) and
still cut beautifully.
The problems we observed were toxicity (we were verrrry careful),the
fact that chloroform does not clear and so care must be taken to mix
contents occasionally to ensure mixing, and most of all the expense
compared to other intermediary agents.
[mailto:firstname.lastname@example.org] On Behalf Of Bryan
Sent: Tuesday, November 29, 2005 11:15 AM
Subject: Re: [Histonet] Re: 70% from NBF
Talking about chloroform use in the past. I trained as a lab tech in
early 60s, and the hospital where I did histology specialist training in
London used chloroform exclusively. The pathologist apparently thought
xylene made tissues too brittle. I have many times dunked my hand in a
litre container of it to get a block out. You wouldn't believe how much
stings, apparently by slightly defatting the skin! I was told this
had done it a few times, of course.
We not only used chloroform without any safety concerns at all, but we
redistilled it to save money. We had an upright, water cooled still
Liebig type condensor. It was heated with an electrical coil in a sand
bath, which surrounded a 2 litre round bottom flask. There was no
cut off for the electricity, and we had to keep an eye on it and switch
off when the flask was almost empty. Of course, being a very attentive
teenager at the time I missed it more often than not. This was usually
announced by a disgusting smell and billows of white smoke. I was never
sure whether the smoke was burning fat and stuff, or phosgene, which is
produced from chloroform. For those not aware, phosgene is one of the
used during World War II as a nerve gas.
I have often wondered whether I became a histotech because I was born
strange, or whether I became strange because of the time I spent
a place like that!
----- Original Message -----
From: "Gayle Callis"
Sent: Tuesday, November 29, 2005 8:23 AM
Subject: [Histonet] Re: 70% from NBF
> Joseph made some excellent points here
> Chloroform is an excellent clearing agent (used it back in the 60's in
> open dip and dunk processors - O.K. so I'm old!) but no one warned us
> about its carcinogenic nature and there were no safety issue
> then. Take his advice!
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