Dorothy
Don't know if this is what you are referring to but...
Some tissue will always stain more deeply with hematoxylin than others.
Tissues that contain large numbers of inflammatory cells such as in the
lamina propria of the digestive tract are often more deeply stained. In
the lamina propria the cells are often much more crowded together.
Cells that carry out a lot of protein synthesis (such as liver cells)
are often much more basophilic than many other cells.
Barry

Those tissues  -----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J
Buesa
Sent: Monday, November 28, 2005 11:12 AM
To: Webb, Dorothy L; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Dark staining

Are the GI biopsies fixed immediately after being taken?
  Do some by any chance air-dry before fixing?
  Are all the sections of the same thickness?
  I would check on these
  Rene J.

"Webb, Dorothy L"  wrote:
  I am wondering what causes some of our tissues to stain with
hematoxylin
darker than others? The pathologist is noticing it mostly on GI
biopsies or colon biopsies, not all of them on the same day either. I
can't believe it is due to improper fixation, as all of them are getting
practically the same amount of time in formalin and it is very adequate,
often more than 24 hours. Any ideas would be appreciated. We use
Gills I hematoxylin and Eosin from sigma.
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