RE: [Histonet] Auto stainer IHC/SS

From:Tom McNemar

The special stains thatI have run have all been very nice except for
masson's trichrome.  We just don't like it.  It is an open system though and
I can use my own reagents and tweak it, just haven't had time.  You cannot
do IHC and special stains at the same time... they have to be separate runs.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Hospital
Newark, Ohio  43055 

-----Original Message-----
From: Lewis, Sarah []
Sent: Wednesday, November 16, 2005 11:10 AM
To: HISTONET (E-mail)
Subject: [Histonet] Auto stainer IHC/SS

Just a quick question in regards to the Autostainer Plus from Dako. I am
currently looking to purchase an Automated Immuno Stainer. I have demoed The
i6000, Mozaic, and the Nemesis.  I will soon demo the Autostainer Plus from
Dako.  They claim to be able to do special stains and IHC. Are any of you
running specials on your Autostainer plus?  Any thoughts on this?  Also,  If
you have any experience with any of the units any feedback would be
appreciated!!!  The i6000 also claims to do specials as well,  I only ran a
few,  I never tried to run IHC and specials together on the same run.(They
have disposable pipette tips which eliminates the cross contamination
issue.)      Any thoughts would be great!!! Thanks in advance!!!           

Sarah E. Lewis 
Childrens Research 
Center for Gene Therapy 
700 Childrens Dr Rm WA3112 
Columbus OH 43205 

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