[Histonet] cryosection bubbles


Dear Histonetters,

When making cryosections of mouse tissues (brain and liver, which is all i
section these days) i get bubble formation between the slide and the

The bubbles are 0.2 -1.0 mm in diameter, and sufficiently large to contact
the blade holder when picking up additional sections on the same slide.

There are no bubbles visible in the OCT, so it seems clear the bubbles are
introduced at the moment the slide is contacting the sections. I get these
bubbles regardless of section thickness (10ums - 25ums).

I'm very satisfied with the sections, but which i could eliminate these
bubbles! Any tips or aspects to pay attention to in this regard are greatly

many thanks in advance,

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