[Histonet] RE: aqueous coverslipping medium
The media you call the "liquid coverslip" is in fact a kind of plastic
polymer that can be mounted up organically after polymerisation. I
experienced that without this additional organic mounting the plastic
polymer will fall off after some years. If this happens you can remove
the plastic completely by soaking the slide in luke warm water.
Then "liquid-coverslip" again.
Although most of those products state in their data sheet that it
should be used without a coverslip, it also works fine WITH a
coverslip. The only thing you should not do is harden at 60-70C as you
normally do (it causes air bubbles!). Just leave the slides at room
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
Date: Wed, 16 Nov 2005 12:40:06 -0500
From: "Monfils, Paul"
Subject: [Histonet] aqueous coverslipping medium
What brand do you prefer for aqueous mounting medium to be used with a
coverslip? I have several good hydrocarbon-based coverslipping media,
also some good aqueous media to be used without coverslips (so-called
coverslip), but I don't have an aqueous coverslipping medium I am
satisfied with. Applications would include immunofluorescence and a
enzyme stains, as well as lipid stains and other techniques that are
compatible with hydrocarbon-based media. Are there any such media
harden after application, to produce a permanent slide?
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