[Histonet] RE: Slide adhesion - toe nail for fungi

From:Malam Jacqueline


We embed the slide of toe nail so it will be cut vertically; then soften the
block surface after trimming in Baker's fluid (10% glycerol in 70% IMS) for
about 20 mins, then coat a slide with a film of gel-chrome alum (0.1% chrome
alum in 1% gelatine), let it dry, mount the section on the slide and dry in
60C oven for a couple of hours then stain. I would avoid a silver stain
though and use PAS or Gomori's aldehyde fuchsin.
Jacqui Malam
Lancaster



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