[Histonet] RE: HRP conjugation
I am wondering if you could use a Mouse-on-mouse detection
system for this purpose. Try Vector MOM kit or Dako ARKit. These kit
are definitely much more simpler than "true" HRP-conjugation.
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
Date: Fri, 18 Nov 2005 13:14:30 -0500
From: "Donin, Nick \(NIH/NCI\)"
Subject: [Histonet] HRP conjugation
This isn't a Histology question, but it relates to antibodies, so I
thought someone could help me. I'm doing a western blot, and I'm
running the protein from mice tumors. I'm using a mouse primary
antibody to stain for a particular antigen, but the problem is that
I apply anti-mouse secondary, it stains everything on the blot
(presumably because the tumor tissue is rich in mouse IgG antibodies).
I thought I could get around this by conjugating my primary antibody
directly to H.R.Peroxidase, thus eliminating the need for a secondary.
My first question is:
1. Does this strategy even make sense?
2. Does anyone have a technique or product for carrying out this
Thanks for all yo! ur help,
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