[Histonet] Movat's Pentachrome question
Does anyone do Movat's Pentachrome stain? I have a researcher who wants to
do it. My question involves the "alkaline alcohol" the procedure calls for.
The protocol says to add ammonium hydroxide drop by drop to 95% ethanol
until the pH reaches 8.0 or higher.
Question 1, a theoretical one - How can you accurately measure pH in a
nearly water-free solution? Presumably an ionic compound would not ionize
very well in such a solution?
Question 2, a practical one - When I put the pH probe into 95% ethanol, the
meter already reads higher than 9.0, even without adding any ammonium
hydroxide. Is this your experience?
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