[Histonet] Fixing question

From:Christian Franci

dear folks, pardon my ignorance but....

It seems that pressure is applied to the processing of tissues to 
ensure evenness and to force out any residual alcohol and/or xylene 
that might over-dry one's delicate samples.
I gather that depending on what machine you have you can apply pressure 
at each step or just at the final paraffin stage ( as in my case).
Here is my question... would applying pressure during the fixation of 
the tissues also be beneficial?
Would it speed up the process therefore minimizing the possibility of 
Do any of you fix tissue under pressure and, if so, how do you go about 
it?  Just curious....



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