[Histonet] Dark staining

From:"Webb, Dorothy L"

I am wondering what causes some of our tissues to stain with hematoxylin
darker than others?  The pathologist is noticing it mostly on GI
biopsies or colon biopsies, not all of them on the same day either.  I
can't believe it is due to improper fixation, as all of them are getting
practically the same amount of time in formalin and it is very adequate,
often more than 24 hours.  Any ideas would be appreciated.  We use
Gills I hematoxylin and Eosin from sigma.
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