Re: [Histonet] teaching histology newbee

From:Geoff McAuliffe

Hi Malcolm:

    I would give a short (15-30 minute) talk about how tissue is 
prepared (fix, dehydrate, clear, embed, section, mount, stain) and show 
a few artifacts (folds, tears, etc). I do think you should briefly 
explain H&E and maybe trichromes and PAS. Definitely explain, with 
illustrations, how immunostaining works.
    In the old days (when I was a student) there might be some 
discussion of how different fixatives can give different 'looks' but now 
that everything is done in buffered formalin (or some secret, commercial 
mixture of who knows what) there seems to be no point in this. Save that 
for teaching microtechnique.
    Good luck!


Malcolm McCallum wrote:

>I will be teaching an undergraduate histology this spring.  For those of you who teach this, what fraction of the course do you devote to techniques versus anatomy?  Do you include pathology, if so, how extensively? How extensively do you require them to understand the differences among stains, and how do you structure your laboratories?  Anything else I should consider?
>Malcolm L. McCallum
>Assistant Professor
>Department of Biological Sciences
>Texas A&M University Texarkana
>2600 Robison Rd.
>Texarkana, TX 75501
>O: 1-903-233-3134
>H: 1-903-791-3843
>Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

Histonet mailing list

<< Previous Message | Next Message >>