Re: [Histonet] crab TEM

From:Rene J Buesa

In 1963 I studied the histology of  the spiny lobster Panulirus argus and the hepatopancreas was one of the tissues I processed. On those days I used a procedure  totally "forbidden" today, namely ethanol (60;70; 80; 95;100) followed by the carcinogen aniline oil (until 
tissue transparent), followed by the also carcinogen benzene before the infiltration. All
steps by hand.
I used the same procedure for fish tissue (Family Lutjanidae) and it worked fine, so if
you have a protocol that works fine on fish I would give it a try (by the way the hepatopancreas
in any decapod crustacean is quite similar).
Hope this will help!
Rene J.

Margaret Horne  wrote:
Hi , 
I am about to process some crab Hipatopancreas and gill 
for TEM . I was contemplating my fish protocol but wondered if 
anyone has done crustaceans.

Margaret Horne ,
Histology Teaching Assistant,
Dept. of B.SC.,
Atlantic Veterinary College, U.P.E.I.,
550 University Ave., Charlottetown,
P.E.I., C1A 4P3 

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