Re: [Histonet] bone fish samples embedded in Historesin Plus

From:Gayle Callis

It may be better to do the sectioning of these samples using a tungsten 
carbide tipped knife , d profile should work.  A regular steel c profile 
knife will not cut through undecalcified bones very well.  It take the 
extremely hard TC knife for this purpose.  Since Historesin Plus is GMA - 
you may want to enter into some discussion about this plastic and 
sectioning with "Patsy Ruegg"  as she has experience 
using this kind of knife with undecalcified bone embedded in GMA.

  At 10:28 AM 11/9/2005, you wrote:

>We are using samples without decalcification because we are studing the bone
>fish development.
>Historesin is glycol metacrylate based (manufactured by Leica).

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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