We are using samples without decalcification because we are studing the bone
fish development.

Historesin is glycol metacrylate based (manufactured by Leica).




----- Original Message -----
From: "Gayle Callis" 
To: "Lina Vieira" 
Sent: Wednesday, November 09, 2005 4:58 PM
Subject: Re: [Histonet] bone fish samples embedded in Historesin Plus


What is Historesin Plus, a plastic? Which one?   At 09:01 AM 11/9/2005, you
wrote:
>Can anyone offer some advice on cutting  bone fish samples embedded in
>Historesin Plus using rigid disposable microtome blades?
>
>We are using the same blades for cut samples embedded in Historesin
>without problems.
>
>But now we have bone fish sample without decalcification embedded in
>Historesin Plus and we canīt get good sections.
>
>Tanks in advance
>
>Lina Vieira
>Universidade do Algarve
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