Before going ahead with Sihler's haemalum method (for peripheral
nerves in cleared whole mounts) consult recent publications:

Wu BL, Sanders I (1994) The Sihler stain: A technique for
studying peripheral nerves in whole-mount specimens. Neuroscience
Protocols Module 4: 83-93.

Wu B, Saunders I (1994) The Sihler stain: a technique for
studying peripheral nerves in whole-mount specimens. In: FG
Wouterlood,  Neuroscience Protocols. Elsevier, Amsterdam. pp.
94-050-07-01 to 94-050-07-10.

Mu LC, Sanders I (1998) Neuromuscular organization of the human
upper esophageal sphincter. Annals of Otology Rhinology and
Laryngology 107: 370-377.

Mu LC, Sanders I (1999) Neuromuscular organization of the canine
tongue. Anatomical Record 256: 412-424.

Gozil R, Kadioglu D, Calguner E, Erdogan D, Bahcelioglu M, Elmas
C (2002) Branching patterns of rabbit oculomotor and trochlear
nerves demonstrated by Sihler's stain technique. Biotechnic &
Histochemistry 77: 21-25.

This is an important method for examining the "gross" anatomy of
small nerves.

Rene: do you have a copy of Bohm & Appel 1907 or of any of
Sihler's papers? If so, I'd be really grateful for bibliographic
details!

John Kiernan
Anatomy, UWO
London, Canada.
______________________________________________________________
Rene J Buesa wrote:
> 
> Hi Dianne:
> Sihler's method was published by Bohm and Oppel in 1907 and is used in pieces of fesh muscle. The solutions are:
> A- water 75 mL + glycerol 12 mL+ acetic acid 12 mL + chloral hydrate 0.75 g
> B- water 75 ml + Ehrlich hematoxylin (1886 formula) 12 mL + glycerol 12 mL + chloral hydrate 0.75 g
> C- 0.1% acetic acid in glycerol.
> Method: pieces of fresh muscle in Sol.A for 18 hours; transfer to Sol.B for 3 to 10 days; transfer to Sol.C untill differentiated, mount in glycerol, seal coverslip with wax.
> Hope this will help!
> Rene J.
> 
> Dianne Holmes  wrote:
> Is anyone familiar with this process to counterstain nerve fibers within a relatively transparent muscle? I need a materials and method list. Can anyone help me?
> 
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