Re: [Histonet] Salivary gland tissue fetal pig problems

From:Geoff McAuliffe

I agree with the other person who responded, you have embalmed material 
and it will never have good morphology.
Also, 30 minutes in formalin won't provide much fixation.
If you want well-fixed salivary glands, you need fresh material that you 
can perfuse or remove, slice into thin pieces, and fix yourself in NBF 
or  Bouin's fixative (for 48 hours or longer).

Geoff wrote:

>Hello again,
>I want to get salivary gland tissue (parotid, submandibular, sublingual) 
>for the students. I thought I would try using the glands from the fetal 
>pigs (sent from Carolina Bio) we use for student dissection. I post-fixed 
>them briefly (~30 minutes) in 10%NBF, followed by 
>dehydration/infiltration. After sectioning, the connective tissue is 
>mostly degenerated and the gland tissue doesn't look that good either. The 
>good archival gland tissue we have is yellowish-colored, maybe it is fixed 
>in something different than NBF. Any suggestions? Thanks.
>Melville B. Vaughan, Ph. D.
>Assistant Professor
>Department of Biology
>University of Central Oklahoma
>Edmond, OK 73034
>Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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