Re: [Histonet] Antigen retrieval on very old tissue

From:John Kiernan

Ask for specimens from known negative and positive 
control brains. Try various antigen retrieval methods.

Process and immunostain + and - controls alongside 
sections of your "very old" material. 

The antigen of interest may not be retrievable
20 years in formalin. 

Just a few thoughts!

John Kiernan
London, Canada.
____________________________________________ wrote:
> What is your recommendation for doing AR on very old brain tissue, while
> minimizing damage?
> I have a brain that has been floating in formalin for 20 years,
> and need to do immuno staining on it.
> Bob Nienhuis
> Neurobiology Research
> UCLA / VA Medical Center
> Los Angeles, CA
> _______________________________________________
> Histonet mailing list

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