Ask for specimens from known negative and positive 
control brains. Try various antigen retrieval methods.

Process and immunostain + and - controls alongside 
sections of your "very old" material. 



The antigen of interest may not be retrievable
 after 
20 years in formalin. 

Just a few thoughts!


John Kiernan
London, Canada.
____________________________________________
nienhuis@ucla.edu wrote:
> 
> What is your recommendation for doing AR on very old brain tissue, while
> minimizing damage?
> 
> I have a brain that has been floating in formalin for 20 years,
> and need to do immuno staining on it.
> 
> Bob Nienhuis
> Neurobiology Research
> UCLA / VA Medical Center
> Los Angeles, CA
> 
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