Re: [Histonet] Antigen retrieval on very old tissue
Ask for specimens from known negative and positive
control brains. Try various antigen retrieval methods.
Process and immunostain + and - controls alongside
sections of your "very old" material.
The antigen of interest may not be retrievable
20 years in formalin.
Just a few thoughts!
> What is your recommendation for doing AR on very old brain tissue, while
> minimizing damage?
> I have a brain that has been floating in formalin for 20 years,
> and need to do immuno staining on it.
> Bob Nienhuis
> Neurobiology Research
> UCLA / VA Medical Center
> Los Angeles, CA
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