Hello, 
I wanted to put my 2 cents worth in about decacifying newborn mouse femurs.  At this age there should not be any calcium yet formed in the bones and do not need decalcifying.  I have done mouse heads at the PO age and I do not have any problem cutting them, and they do not need decalcified.

Rhonda Allen BA HT(ASCP)HTL, QIHC
Stowers Institute
Histotechnology Specialist II		
1000 E. 50th street
Kansas City, Missouri 64110
rra@stowers-institute.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pex
Sent: Wednesday, November 02, 2005 7:52 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] decalcification


Dear all,
 
I have a question about decalcification. I have got some bone tissues 
(such as femur) from newborn pups, I would like to know if they need to 
be decalcified before paraffin embedding.
 
Thanks a lot.
 
Guofeng


		
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