Well said Pam and Gayle!
I know that every time I have a problem, my first thought is "ask histonet" not because I am lazy or because I can't look it up.  I think first of histonet because of the diversity of our listmembers and their experience.  Just because a publication lists the steps of a protocol, doesn't mean it will work.  I would rather get my info from the ones who make these protocols work.
On the other hand, every time I am about to post a query, I have to read it 6 times to make sure there isn't anything in the post that I will be chastised for.  While I agree that we should not "post with reckless abandon", I do not think we should be terrified of backlash for asking how to do something we've never done before, especially if it affects patient care, whether directly or indirectly.
 
Well, I am going to put on my bullet proof vest!
Thanks for listening,
Jennifer
 
 
 
Jennifer Hofecker, HT (ASCP)
Vanderbilt University Medical Center
Division of Neuropathology
(615) 343-0083
(615) 343-7089 fax

Thank You Gayle!!  It is sometimes easy to forget not everyone has access
to the Internet or a way to even place a message on HistoNet.  We are
attempting to assist people who are learning and while some may seem to be
using HistoNet as way to avoid looking anything up the majority are asking
questions that are not readily accessible or in an area  like murine animal
models that is more difficult.  I have no problem with this and will answer
most questions off line if it is a more esoteric  question.  I am surprised
that the person with the issue about Rene's assistance for her friend is
also the same Rene who has been asking all of us to contribute to Tricks of
the Trade.

Pamela A Marcum
Special Procedures Manager
Bone Histology
UPENN Vet School


At 05:33 PM 11/9/2005, Gayle Callis wrote:
>Rene,
>
>Sorry to disagree, and I certainly did not understand what YOU meant by
>"changing his ways"?  I couldn't see many changes here, other than some
>refinement of his IHC method.  Many people working with murine CD marker
>IHC often need a little help with murine  animal model IHC.   Since our
>lab works with murine CD marker IHC approx. 95% of the time, on frozen
>sections etc per the inquiry, we are happy to sleuth problems.  The
>questions were valid ones for learning/refining of his techniques and
>being a mousie person, I didn't have a problem with the inquiry.
>
>Some people (including a lot of experts in our field) do NOT want to be on
>Histonet and second hand messaging certainly doesn't bother me as long as
>more details are provided.  I frequently ask questions on Histonet to
>access information for others in my department - that's part of my job.
>Your colleague is welcome to email privately if you or they wish.
>
>Gayle Callis
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367
>406 994-4303 (FAX)
>
>
>
>
>
>
>- At 02:28 PM 11/9/2005, you wrote:
>>With all due respect I think that your colleague's procedure is a royal mess.
>>On the other hand it seems that he is not very willing to change his
>>ways, so the only thing I have to tell you (for him) is: good luck! He
>>should be the one doing the asking, and not you!
>>Rene J.
>
>Gayle Callis
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367
>406 994-4303 (FAX)
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
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