[Histonet] crumbly femurs
I am trying to section some mouse legs. The bones were fixed/decalcified for one week then quick frozen in OCT and put at -20C where they have been for about 2 months. Regardless of how thick I cut the section (5-20 microns), whether I cut free hand, use our cryojane or the old fashioned scotch tape type of tape transfer, there is little/no visible bone in the section. Just OCT and an empty space where bone would be. If I cut without tape I can see the bone crumble as I cut. This seems to be a catastrophic failure of some sort and may be beyond any type of help at this point but if anyone has any ideas as to why this is happening I would be happy to hear from you.
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