Re:Marking ink for maintaining orientation RE: [Histonet] freezing mouse eyes

From:slarkham@qltinc.com






Yup i am with you on the marking with ink Gail, it works extremely well for
us and then orientation isnt an issue, we use a dissecting microscope to
put the ink on and then freeze using neg 50 (better than OCT in my
eyes...heh no pun intended) in a cryomould and freeze on the surface of
liquid nitrogen


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