Re: [Histonet] supravital stains

From:John Kiernan

Dear Jenny Oblander,

You may be way out on the wrong track, looking
for a dye that does not really exist. 

Rhodanile blue was a product made and sold by 
Edward Gurr in the 1960s. It was supposedly 
formed by chemical combination of
rhodamine B with nile blue A. For an
intelligent but inconclusive discussion of
this product, see the 9th edition (1977) of
Conn's Biological Stains, page 411. 

Rhodanile blue is not mentioned in the 10th
edition of Conn's because there's probably
no such dye, even if some suppliers sell
something with that name. Anyone intending
to use a product called rhodanile blue should
find and read all the references in the 9th
edition of Conn's as well as Gurr's books.
These books are in public and university
libraries, and on the shelves of labs that
contain stainthusiasts. 

The reference for staining Heinz bodies (here
cited from Lillie's 9th edn of Conn's) is
Simpson GF et al 1970. Stain Technol. 45:221-223.
Look up and evaluate this paper. Did Simpson
et al know what they were doing? 

Querky dye methods may not be the best way 
to stain Heinz bodies.  If you can explain
what they contain, you will probably get an
abundance of replies from histonetters.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
Jenny Oblander wrote:
> Hi again,
> Does anyone have a procedure for Rhodanile blue.I'm looking for Heinz
> bodies. Thanks Jenny
> J.Oblander, HT (A.S.C.P.)
> Comparative Medicine
> Oklahoma Medical Research Foundation MS#32
> 825 NE 13th St.
> Okc,Ok 73104
> 405-271-7083
> _______________________________________________
> Histonet mailing list

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