Re: [Histonet] gliut/isopropyl alcohol
In a message dated 11/30/2004 12:12:08 PM US Mountain Standard Time,
BMolinari@heart.thi.tmc.edu writes:
I received samples that have been fixed in a 1%glut / 20% isopropyl
alcohol solution for paraffin processing. I have never heard of this
fixative, I know alcoholic formalin but never alcoholic glutaraldehyde.
Are any of you familiar with this fixative?
Thank you.
Betsy Molinari HT (ASCP)
Hi Betsy,
Is the specimen perhaps a heart valve made from pig tissue??
The only time I've heard of alcoholic glutaraldehyde is for pretreating pig
valves before they're implanted in humans. There's some kind of relationship
between alcohol and glut, and preventing calcification of the valve. Can't
remember the details right now, but you have to treat the valve with one of those
reagents before using the other.
Just a thought...
Cheers,
Bob Chiovetti
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