Re: [Histonet] Whole mount prostate specimens

From:"Gudrun Lang"

Hi Angie,
In our lab we do not perform whole mounting, but use the macro-cassettes and
slides, that are 4 times larger than the standard ones.
The doctor cuts the protstata in mostly 6 slices, after fixation for at
least two days. Tissue processing requires double times than normal.
We cut them on routine sliding microtomes (Microm) with a special
blockholder. The cutting is not difficult.

hope this helps
Akh Linz, Austria

----- Original Message ----- 
From: "Angela Bitting" 
Sent: Monday, November 22, 2004 8:53 PM
Subject: [Histonet] Whole mount prostate specimens

> I have been asked to research what type microtome, slides, coverslips,
> knives I will need to begin cutting whole mount prostates.
> If anyone out there is doing this in the clinical hospital setting,
> could you help me out. Any procedures, hints, resources,....anything
> will help.
> What I'd really like to do is see someone actually cutting these type
> specimens. Get some one-on-one training. I'm in central Pennsylvania if
> anyone in the area  wants to offer me some personal instruction.
> Thanks folks!
> Angie
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center 23-00
> 100 N Academy Ave.
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916
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