Re: [Histonet] Water contamination
From: | Jose Luis Palazon Fernandez |
In my lab we normally use 95 % EtOH before absolute alcohols, instead of the 70 % you use, without any problem. Maybe your contaminating agent is 70 % EtOH, try changing this step with a 95 % one
José Luis
El dia 27/11/2004 19:32 usted envio el siguiente mensaje:
>Date: 27 de Noviembre de 2004 19:32:07
>From: Paula Pierce
>Subject: [Histonet] Water contamination
>To: histonet@lists.utsouthwestern.edu
>
> First, you SLICE meat. Histotechs SECTION tissue.
>
> 70% EtOH is 30% water. You are probably getting too much water carryover into your 100%. Make sure the 100% EtOH is 100%. If the solutions are fresh, 10 to 20 dips each is enough time for dehydration before xylene and coverslipping.
>
>
> Paula Pierce, HTL(ASCP)HT
>
> Excalibur Pathology, Inc.
> 631 N. Broadway
> Moore, OK 73160
> 405-759-3953
> contact@excaliburpathology.com
> www.excaliburpathology.com
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Universidad de Oriente-Isla Margarita-Venezuela
actualmente en: Instituto de Ciencias Marinas de Andalucia
Puerto Real, Cádiz, España.
email: jluis.palazon@icman.csic.es
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