Re: [Histonet] Re: Ammonia water pH and other bluing solutions

From:"Jackie M O'Connor"

Anyone remember using saturated lithium carbonate?  I could probably use a 
little lithium right now . . . . . 

Correct me if I'm wrong (Pandora's box) but isn't the "bluing" step just 
bringing the slides BACK to a neutral pH after treating them with acid 
which makes them purple-ish?  I like the ammonia because it 'seems' to 
make the nuclei sharper and instantaneously - Li2Co3 and PBS seemed to 
make them "blah".(Perception is in the eye of the beholder)   Running tap 
water will make the same miracle happen, but it takes longer.

"Johnson, Teri" 
Sent by:
11/30/2004 12:51 PM

        To:     "Histonet" 
        Subject:        [Histonet] Re: Ammonia water pH and other bluing solutions

Way back in the day, Brigati was using PBS on his immunostainer to blue
after the hematoxylin counterstain.  I have since used PBS to blue my
slides instead of ammonium hydroxide/water.  It's cheap, and very easy
on the sections, and I have plenty of it on hand.

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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