Re: [Histonet] RE: Envision-AP
I've been using the Dako Envision AP kit for years but (after much trial
and error), I have use the New Fuchsin Kit from BioGenex as my label.
Works great when used properly and it is permanent. Avoid xylene after
dehydration, instead usa a non-aliphatic clearing agent (I use Richard
Allan's Clear Rite 3). Coverslip with your choice of media. The
results are a nice bright red which doesn't seam to fade at least not in
the last 7-8 years that I've used it.
Ive not used the "PowerVision that Chris mentioned and so can't comment
on it. I would like some information on it (better yet a trial).
C.M. van der Loos wrote:
>I was thinking the same way as Gayle did. But apparently this wasn't the problem. I wondered what AP chromogen you are using? I have very good results using the newest Dako Liquid Permanent Red substrate kit.
>The KPL universal blocker Gayle suggested I don't know. However, I am familiar with a similar Dako product called Dual Endogenous Enzyme Block. This blocker also applied before the IHC procedure also effectively destroyed some of my epitopes resulting in a bit too clean staining result indeed.... So be careful with this stuff.
>Furthermore, I am using PowerVision-AP with great results.
>Hope this helps.
>Chris van der Loos, PhD
>Dept. of Pathology
>Academical Medical Center
>NL-1105 AZ Amsterdam
>phone: +31 20 5665631
>----- Original Message -----
>>From Ian Montgomery
>Date Thu, 18 Nov 2004 17:22:06 +0000
>Subject Fwd: Re: [Histonet] Envision.
>>Question: what buffer are you using? If you use PBS, then you will have
>>phosphate ions available for AP, TRIS buffered saline is preferred with AP
> I'm using TBS as suggested by Dako. With and without Tween, but
>still the same result.
>>At 08:52 AM 11/18/2004, you wrote:
>>> I wonder if anyone can offer any hints and tips for Dako
>>>Envision. Localisation is fine, HRP is clean but alkaline phosphatase,
>>>despite my efforts, is still giving me quite a lot of background. This
>>>manifests itself as a fine red ppt. over the specimen.
>>>Dr. Ian Montgomery,
>>>Graham Kerr Building,
>>>Institute of Biomedical & Life Sciences,
>>>University of Glasgow,
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