Re: [Histonet] IHC on Sections Processed for EM?

From:Geoff McAuliffe

Hi Michael:

    As usual, it "depends on the antigen". There are a few antigens that 
survive glut and osmium, I know that GFAP can be made to work on some 
astrocytes if the osmium is removed. Other than that, I would think a 
literature search for the antigen in question is the best first step. 
Maybe someone has made it work? Of course, the Epon will have to be etched.


Michele French wrote:

> Good Morning Histonet! A colleague of mine did some EM work and found 
> something interesting. Our pathologist wanted me to try to do some IHC 
> to further characterize what is present in the section. Our EM person 
> said it would never work. I did not think it was possible either, but 
> I thought I would ask anyway. Has anyone tried doing an immunostain on 
> plastic (Epon) sections from tissue fixed and processed for EM? I am 
> always up for a challenge, but I am really busy right now and don't 
> want to waste my time if there is really no hope! Thanks in advance,  
> Michele French
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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