Re: [Histonet] F4/80 on formalin fixed frozen spleen
If you have to do retrieval on frozen section fixed with formalin, then you
may lose section.
Why do just do paraffin, and then retrieval for F4/80. Other labs have
gotten this to work, and has been posted on Histonet - check out archives
At 11:40 AM 11/24/2004, you wrote:
>Does anyone know if F4/80 can work on formalin fixed frozen mouse spleen
>for IHC without antigen retrieval?
>Also, thanks to everyone for their suggestions re. mouse B-cell markers.
>NIH, NICHD, CBMB 301.435-8417
>Building 18T, room 101 301.402-0078 fax
>Bethesda, MD 20892
>Histonet mailing list
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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