If you have to do retrieval on frozen section fixed with formalin, then you 
may lose section.

Why do just do paraffin, and then retrieval for F4/80.  Other labs have 
gotten this to work, and has been posted on Histonet - check out archives 
for information.

At 11:40 AM 11/24/2004, you wrote:
>Does anyone know if F4/80 can work on formalin fixed frozen mouse spleen 
>for IHC without antigen retrieval?
>
>Also, thanks to everyone for their suggestions re. mouse B-cell markers.
>
>Thanks,
>Sharon
>--
>Sharon Cooperman                     
>NIH, NICHD, CBMB                     301.435-8417
>Building 18T, room 101               301.402-0078 fax
>Bethesda, MD 20892
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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