Re: [Histonet] Elution reagent for Double Staining
Talk to Chris van der Loos - he has done this and actually feels this does=20
NOT work very well. There are other ways to get excellent results with
double IHC and not use elution. He can be reached at "C.M. vander Loos"
. There are problems with elution. One is the=20
antibody is bound so strongly to antigen, it will not elute. Another
is damage to antigens with elution reagents. Chris is a master at double=20
staining and taught this in Toronto this past year - look for him to do
this again in Fort Lauderdale, NSH symposium 2005 - he did discuss elution=20
and how to get around it.
A good visit with him may help you with your double staining.
At 07:33 AM 11/23/2004, you wrote:
>Anyone out there willing to share what they use for elution reagent during=20
>double immunostaining using peroxidase and phosphatase systems
>together? I have looked and gotten a variety of answers, I would like to=20
>know what actually works for people. Thanks in advance -
>Amy S.Porter, HT(ASCP)
>Michigan State University
>Department of Physiology
>Division of Human Pathology
>College of Human Medicine
>Do you Yahoo!?
> Meet the all-new My Yahoo! Try it today!
>Histonet mailing list
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