Re: [Histonet] Ammonia water pH
|From:||"SMITH,REBEKAH FELICIA" |
I agree with Jackie, I use a Coplin jar with distilled water and
about 3 drops of 30% ammonium hydroxide, and then rinse in tap
water twice. i've never had problems and it produces a rather
pretty blue for me.
On Tue Nov 30 10:35:55 EST 2004, Jackie M O'Connor
> Maybe I'm being extremely simple-minded - but I just fill up a
> 200 ml staining jar with tap H20, and add a couple-three drops of
> 28%. When it doesn't smell like ammonia anymore, I make new
> stuff. How's that grab ya for QA? "Tim Webster"
> Sent by: firstname.lastname@example.org
> 11/30/2004 09:30 AM
> cc: Subject: [Histonet] Ammonia water pH
> Hi All,
> I'm curious to know what people's procedures are for making and
> using ammonia water as a bluing agent in regressive H&E's.
> Currently, our methodology is to make the ammonia water up daily
> from a 28% stock bottle, and the pH is about 11. My feeling is
> that 11 is way to high and that 9-10 is plenty alkaline to blue
> sections adequately. Additionally, I'm concerned about strong
> alkaline solution increasing the likelihood of sections washing
> off or wrinkling. Sheehan & Hrapchak call for a "weak" alkaline
> solution in "Theory and Practice of Histotechnology". Also, I can
> see no reason not to make a working solution weekly/monthly and
> simply empty and refill the Ammonia water station. The pH
> doesn't appear to drop off over time.
> Thanks for your time, and have a great week! Tim
> Tim Webster
> Histology Specialist
> Northwestern Medical Center
> 133 Fairfield Street,
> St Albans, VT 05478
> (802) 524-1070 (x4349)
>> Statement of
>> This message and
> attachments are from NMC and intended only for the addressee(s).
> The information contained may include privileged or other
> confidential information. Unauthorized review, forwarding,
> printing, copying or distribution is strictly prohibited.
>> If you should
> this message in error, please delete it and notify the sender.
> Thank you.
> Histonet mailing list
> Histonet mailing list
"You are a child of the universe, no less than the trees and the
You have a right to be here and whether or not it is clear to you,
no doubt the universe is unfolding as it should. Therefore be at
peace with G-d, whatever you conceive Him to be. And whatever your
labors and aspirations,in the noisy confusion of life, keep peace
in your soul.-Max Ehrmann,"Desiderata"
Histonet mailing list
<< Previous Message | Next Message >>