RE: [Histonet] blotches in sections

From:Tony Henwood


My first impression is that there is water on the sections. You may possibly
have to prolong your dehydration and clearing prior to coverslipping. You
could decoverslip, remove the mountant in xylene and complete the
dehydration in ethanol and then (via xylene or its substitute) recoverslip.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Pool, Madeline [] 
Sent: Friday, 26 November 2004 6:15 AM
Subject: [Histonet] blotches in sections

I'm cutting 10 um sections from paraffin embedded mouse brain, spinal
cord, and optic nerve and staining them with Luxol Fast Blue. In the
brain sections, I'm seeing some splotchy darker background. I'm
wondering if maybe I didn't dry the slides enough after sectioning or
maybe heated it too much or something else entirely. If anyone has seen
things like this before and can tell me what I should do to fix it,
please let me know. I've put a picture of a section up at: (Don't worry about the
tearing, I was testing out bad sections.)

Thanks in advance,

Madeline Pool
Ottawa Health Research Institute
501 Smyth Road
Ottawa, Ontario
K1H 8L6
fax: (613)737-8023

Histonet mailing list

This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 

Histonet mailing list

<< Previous Message | Next Message >>