I don't think fiddling with a few percentages of alcohol in the initial
steps of dehydration makes any difference, bung it straight in 100%, it'll
get diluted after a few batches.

Change your last alcohol more regularly or have a 'drip bottle' of alcohol
and, if you are manual staining, give the slides a quick wash of fresh
alcohol before entering the first xylene. That's a point, did you say you
were manual staining or not?

Kemlo Rogerson
Cellular Pathology Manager
East Lancashire Hospitals NHS Trust
DD. 01254-294162
Mobile 0774-9754194
 

-----Original Message-----
From: Jose Luis Palazon Fernandez [mailto:jluis.palazon@icman.csic.es] 
Sent: 29 November 2004 16:59
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Water contamination [Scanned]

In my lab we normally use 95 % EtOH before absolute alcohols, instead of the
70 % you use, without any problem. Maybe your contaminating agent is 70 %
EtOH, try changing this step with a 95 % one



José Luis





El dia 27/11/2004 19:32 usted envio el siguiente mensaje:

>Date: 27 de Noviembre de 2004 19:32:07

>From: Paula Pierce  

>Subject: [Histonet] Water contamination 

>To: histonet@lists.utsouthwestern.edu

>

> First, you SLICE meat. Histotechs SECTION tissue.

>  

> 70% EtOH is 30% water. You are probably getting too much water carryover
into your 100%. Make sure the 100% EtOH is 100%. If the solutions are fresh,
10 to 20 dips each is enough time for dehydration before xylene and
coverslipping. 

> 

> 

> Paula Pierce, HTL(ASCP)HT

> 

> Excalibur Pathology, Inc.

> 631 N. Broadway

> Moore, OK 73160

> 405-759-3953

> contact@excaliburpathology.com

> www.excaliburpathology.com

> _______________________________________________

> Histonet mailing list

> Histonet@lists.utsouthwestern.edu

> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

> 







Universidad de Oriente-Isla Margarita-Venezuela

actualmente en: Instituto de Ciencias Marinas de Andalucia

Puerto Real, Cádiz, España.

email: jluis.palazon@icman.csic.es

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