RE: [Histonet] RE: IHC on tissues processed for EM

From:"pam marcum"

Thank you, Barry. 

 We all have days when even the simplest answers to common questions just
aren't there and we ask the community of histologists we all belong to for
help.  Other times looking up the answer with the sources available can be
best.  We have to determine what we need and use those resources well.  I
think sometimes we use the easiest way to avoid taking the time to learn and
everyone can be guilty of this at one time or another.  It is only an issue
when we take the easy way out every time.  

Often I see questions or requests for help when it is clear the person is in
trouble, needs help and isn't even sure how to ask or what to ask.  In that
position they may not even know where to look as they are given a project
and told do it with no help.  Until we can all wiggle our noses and make it
happen we should look at the community as place for help and not abuse it=2E 
I only have a problem when it is a student who clearly is asking us as a
group to make it too easy for them to ask and not learn.  The dangers of
only knowing our field by rote or to repeat what they have read or heard is
just not acceptable when the patient is at risk for not getting the best
care.  I feel the same way about research projects as the responsibility of
the technician to make the effort to learn and grow everyday.

Pam Marcum


-------Original Message-------


From: Rittman, Barry

Date: 11/29/04 17:11:42

To: histonet

Subject: RE: [Histonet] RE: IHC on tissues processed for EM


Dawn et al.

I do not think that this is a totally black and white problem.

I think that it is entirely appropriate to ask people on Histonet for their
opinion on a specific technique as there are many experts in the various

While I agree that there are no stupid questions, I believe that the intent
of the writer was not to deride the individual but to suggest that they
first try to search out the information before asking the question.  I have
taught students for many years and feel that more and more I am being asked
to spoon feed individuals rather than to get them to learn the material. It
is easier to provide the correct answer and considerably  more difficult to
motivate people to work out the solution for themselves (if they can) i.e=2E I
believe that a ready made answer is usually not in the best interests of
learning for that individual.

When I was training (and I feel that I still am) while I could go and ask an
"expert" next door for the answer, I always tried to find the answer out by
myself first. This was, I believe, to my benefit as I was able not only to
ask a more pointed question of the expert but also ask some important follow
up questions.  I believe that it is a much more satisfying approach.


On the other side of the coin you have to realize that some of the
individuals asking these questions may not have the resources available to
them or even know where to begin to find out the information.

I would suggest to those who wish to ask questions that you first try to
research the material if you can as you will then be able to ask questions
specific to your needs. You will benefit both ways.




-----Original Message-----

[] On Behalf Of hymclab

Sent: Monday, November 29, 2004 3:31 PM

To: 'Gudrun Lang'; Histonetliste

Subject: RE: [Histonet] RE: IHC on tissues processed for EM


I too think that no question should be lableled as "easy".  There are no

stupid questions.  I recently went through an issue about a stain that I

have been doing for over 20 years.  Couldn't figure out what was wrong.

Technical services at two companies were stumped until someone came up with

a possible answer.  I tested it and they were correct.  Someone else on the

Histonetlist just had the same problem and I responded with what we found to

be our problem.

Then I got slammed by someone who couldn't have even read the whole message.

I was answering someone'e problem about their mucicarmine stain not working.

In my response to this person I used the terms Mayer's mucicarmine and

Weigert's Hematoxylin.  This other person that slammed accused me in living

in the middle ages for using Mayer's Hematoxlyin and Weigert's hematoxlyin

and said I should try Gill's hematoxylin.  I didn't say I used Mayer's

hematoxylin, I was referring to the name of the Mucicarmine stain.  Secondly

I had never heard of using Gill's in place of Weigert's in the Mucicarmine

stain.  I e-mailed him back I thought maybe he was confused---he said he

wasn't and he said that he'd never even done a Mucicarmine stain.  I rest my


Things like that make you want to be a lurker instead of trying to help






-----Original Message-----

From: Gudrun Lang []

Sent: Thursday, November 25, 2004 3:09 AM

To: Histonetliste

Subject: Re: [Histonet] RE: IHC on tissues processed for EM




I feel about saying some words about your message.

You complain, that members of the histonetlist have no basic knowledgment=2E

But what is basic? For me, in a routine surgery histolab, EM is very, very

special for example. I belong to those people, that work in a small corner

of the whole histological techniques. But I am interested and want to learn.

You say, people have to use databases, text books etc. In my case I have no

access through my lab to those things and they are'nt cheap (and first you

have to know where to look). I am very happy about the histonetlist. Here=0D
are specialists, experts from every field and also "workmates". Please be=0D
tolerant with "easy" questions and problems, perhaps the person considers=0D
the problem more difficult than you know. friendly greetings Gudrun Lang




----- Original Message -----

From: "Laszlo Komuves" 


Sent: Wednesday, November 24, 2004 8:44 PM

Subject: [Histonet] RE: IHC on tissues processed for EM



There are hundreds, if not thousands of papers demonstrating successful

localization of intracellular antigens at EM or LM level using tissue

samples processed conventionally for EM (i.e. PFA/GA fixation, Os

postfixation, epoxy resin embedding).


Look  for the now classical papers by Roth and Bendayan or their early

reviews. Also the EM textbooks published in the 80's have plenty of



And let me also add a personal comment of frustration: Histonet used to be

about sharing experience and wisdom, mentoring and teaching those have were

(are they still?) eager to learn or perfect their skills. I personally

learnt/benefited from many messages posted. Now people are asking advice

about coverslip sizes? So thanks to the curse of the Internet, journal

articles and text books are used only by a dedicated few, electronic

databases (libraries, PubMed, journal archives, reagent search sites, even

Google) are not searched, and now even the vendor catalogs are not opened,

because on the Histonet somebody will respond? How will users differentiate

between solid information (based on scientific knowledge, practical

experience) or just plain ignorance? Where is critical thinking? What

happened to a once honorable guild of skilled craftsmanship, when members of

the community are  lacking elementary knowledge (just a few recent

hair-raising topics: H&E staining, pH, and the list goes on and on)?  And of

course we/they are constantly offended/complain about the lack of

recognition by our/their peers.


Feel free to get angry with me. I certainly welcome arguments and

criticism.. As for me, I go back to find something useful in one of my



Laszlo Komuves


FROM:  László G. Komuves PhD

Senior Principal Scientist,

Manager, Microscopy Core Laboratory



650 Gateway Blvd., South San Francisco, CA 94080

Phone: (650) 246-6905, Fax: (650) 624-7540



Date: Tue, 23 Nov 2004 08:02:24 -0500

From: Michele French 

Subject: [Histonet] IHC on Sections Processed for EM?


Message-ID: <>

Content-Type: text/plain; format=flowed; charset=ISO-8859-1


Good Morning Histonet! A colleague of mine did some EM work and found

something interesting. Our pathologist wanted me to try to do some IHC to=0D
further characterize what is present in the section. Our EM person said it

would never work. I did not think it was possible either, but I thought I=0D
would ask anyway. Has anyone tried doing an immunostain on plastic (Epon)=0D
sections from tissue fixed and processed for EM? I am always up for a

challenge, but I am really busy right now and don't want to waste my time if

there is really no hope! Thanks in advance,  Michele French




Histonet mailing list




Histonet mailing list



Histonet mailing list



Histonet mailing list
Histonet mailing list

<< Previous Message | Next Message >>