RE: [Histonet] RE: IHC on tissues processed for EM

From:"Koelling, Ray"

Not only would I not get angry but more than happy to meet you and buy you a
Seattle seafood meal for saying it if you ever venture up this way.  And I'm
not kidding.

Raymond Koelling
Resaerch Scientist, Pathology
Amgen Corp.
Seattle, WA 98119

-----Original Message-----
[]On Behalf Of Laszlo
Sent: Wednesday, November 24, 2004 11:44 AM
Subject: [Histonet] RE: IHC on tissues processed for EM

There are hundreds, if not thousands of papers demonstrating successful
localization of intracellular antigens at EM or LM level using tissue
samples processed conventionally for EM (i.e. PFA/GA fixation, Os
postfixation, epoxy resin embedding).

Look  for the now classical papers by Roth and Bendayan or their early
reviews. Also the EM textbooks published in the 80's have plenty of

And let me also add a personal comment of frustration: Histonet used to be
about sharing experience and wisdom, mentoring and teaching those have were
(are they still?) eager to learn or perfect their skills. I personally
learnt/benefited from many messages posted. Now people are asking advice
about coverslip sizes? So thanks to the curse of the Internet, journal
articles and text books are used only by a dedicated few, electronic
databases (libraries, PubMed, journal archives, reagent search sites, even
Google) are not searched, and now even the vendor catalogs are not opened,
because on the Histonet somebody will respond? How will users differentiate
between solid information (based on scientific knowledge, practical
experience) or just plain ignorance? Where is critical thinking? What
happened to a once honorable guild of skilled craftsmanship, when members of
the community are  lacking elementary knowledge (just a few recent
hair-raising topics: H&E staining, pH, and the list goes on and on)?  And of
course we/they are constantly offended/complain about the lack of
recognition by our/their peers.

Feel free to get angry with me. I certainly welcome arguments and
criticism.. As for me, I go back to find something useful in one of my

Laszlo Komuves

FROM:  László G. Komuves PhD
Senior Principal Scientist,
Manager, Microscopy Core Laboratory

650 Gateway Blvd., South San Francisco, CA 94080
Phone: (650) 246-6905, Fax: (650) 624-7540

Date: Tue, 23 Nov 2004 08:02:24 -0500
From: Michele French 
Subject: [Histonet] IHC on Sections Processed for EM?
Message-ID: <>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1

Good Morning Histonet! A colleague of mine did some EM work and found 
something interesting. Our pathologist wanted me to try to do some IHC 
to further characterize what is present in the section. Our EM person 
said it would never work. I did not think it was possible either, but I 
thought I would ask anyway. Has anyone tried doing an immunostain on 
plastic (Epon) sections from tissue fixed and processed for EM? I am 
always up for a challenge, but I am really busy right now and don't want 
to waste my time if there is really no hope! Thanks in advance,  Michele 

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