RE: [Histonet] Paraffin processing explant cultures

From:"Johnson, Teri"

Liz is a genius.  I called Millipore and their customer service guy,
Rich, told me I could paraffin process and section these with no
problems.  Why didn't *I* think of doing this?  Sheesh...


-----Original Message-----
From: Elizabeth Chlipala [] 
Sent: Tuesday, November 30, 2004 4:03 PM
To: Johnson, Teri; 'Histonet'
Subject: RE: [Histonet] Paraffin processing explant cultures


I have processed explant cultures with the Millipore MillicellT CM which
is a polytetrafluoroethylene based polymer.  I process these as any
other tissue.  Millipore has a great web page in which you can ask
questions.  I would give them a call about the particular filter that
you are using. 


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of Johnson,
Sent: Tuesday, November 30, 2004 2:14 PM
To: Histonet
Subject: [Histonet] Paraffin processing explant cultures

Is anybody doing paraffin processing on explant cultures and can give me
some tips on increasing my chances for success?  The culture plate
inserts are Millicell polycarbonate and my question is whether they can
survive tissue processing and paraffin sectioning.  If not, what are
your recommendations for doing these?
Thanks in advance!

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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