Tom

I did some work in the past with RDI's goat-anti-Luciferase.  But from
what I remember it was a bit difficult to work with and I was not that
pleased with the results.  I believe I had some issues with non-specific
staining.  I do have a protocol that you can start with and some images
if you would like them.  The sections I used were paraformaldehyde fixed
paraffin embedded mouse lung, kidney and liver.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
t-kuzniar@md.northwestern.edu
Sent: Tuesday, November 16, 2004 3:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Luciferase antibody

Hi everyone, 

I am looking for a nice anti-luciferase antibody for
immunohistochemistry for formalin-fixed,
paraffin-embedded sections of the lung. 

Tom Kuzniar
t-kuzniar@md.northwestern.edu



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