RE: [Histonet] Counterstain for Von Kossa

From:"Elizabeth Chlipala"

Frederik

Nuclear Fast Red is the counterstain that I use when I run a Von Kossa.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fredrik
Johansson
Sent: Monday, November 29, 2004 10:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Counterstain for Von Kossa

Hi,

†

I am going to stain 5 um paraffin sections of mouse arteries for 
calcium (Von Kossa) and need a counterstain. Iím new in this area and 
would appreciate if some one could tell me were to find a protocol for 
this. I have looked in the archive and found a protocol for bone 
sections (toluidine blue) can I use the same protocol for my mouse 
arteries?

†

Thanks

†

Fredrik Johansson

Department of Pathology

UW

Seattle


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