Personally, I don't like the Bodian stain. For most nerves, the Holmes
technique works well on the first try (Kiernan, HISTOLOGICAL AND
HISTOCHEMICAL METHODS, 3rd ed., p. 366).  
  You cannot beat Kiernan's physical developer method for really tiny nerves
(Kiernan, HISTOLOGICAL AND HISTOCHEMICAL METHODS, 3rd ed., p. 371). It can
detect single axons.  Kiernan's method takes practice, but it is worth
learning.  After 3 tries, you should have mastered it.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
            Podiatric Medicine and Surgery
Miami Shores, Florida


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Delorise
Williams
Sent: Thursday, November 18, 2004 4:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bodian silver stain



Hi Everyone, Would like to please talk to someone that has had good
results with the Bodian stain. Also would like to know the most common
stain or method being used in the labs today to demonstrate nerve
fibers, endings and neurofibrils? I work in research primarily with
animal tissue but presently am working with human nasal turbinate
tissue.

Delorise Williams
CIIT Centers for Health Research 
PO Box 12137
Research Triangle Park, NC  27709
(919) 558-1200	
Voice Mail-(919) 558-1252
Fax-(919) 558-1300

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Barry University - Miami Shores, FL (http://www.barry.edu) 

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