RE: [Histonet] Ammonia water pH[Scanned]

From:Kemlo Rogerson

Bang on! Weak is 0.2 to 0.3% NH40H, anymore and the sections disappear.

Kemlo Rogerson
Cellular Pathology Manager
East Lancashire Hospitals NHS Trust
DD. 01254-294162
Mobile 0774-9754194

-----Original Message-----
From: Jackie M O'Connor [mailto:Jackie.O'] 
Sent: 30 November 2004 15:36
To: Tim Webster
Subject: Re: [Histonet] Ammonia water pH[Scanned]

Maybe I'm being extremely simple-minded - but I just fill up a 200 ml 
staining jar with tap H20, and add a couple-three drops of 28%.  When it 
doesn't smell like ammonia anymore, I make new stuff.  How's that grab ya 
for QA? 

"Tim Webster" 
Sent by:
11/30/2004 09:30 AM

        Subject:        [Histonet] Ammonia water pH

Hi All,

I'm curious to know what people's procedures are for making and using 
ammonia water as a bluing agent in regressive H&E's.
Currently, our methodology is to make the ammonia water up daily from a 
28% stock bottle, and the pH is about 11.  My feeling is that 11 is way to 
high and that 9-10 is plenty alkaline to blue sections adequately. 
Additionally, I'm concerned about strong alkaline solution increasing the 
likelihood of sections washing off or wrinkling.  Sheehan & Hrapchak call 
for a "weak" alkaline solution in "Theory and Practice of 
Also, I can see no reason not to make a working solution weekly/monthly 
and simply empty and refill the Ammonia water station.  The pH doesn't 
appear to drop off over time.

Thanks for your time, and have a great week! 


Tim Webster
Histology Specialist
Northwestern Medical Center
133 Fairfield Street,
St Albans, VT 05478
(802) 524-1070 (x4349)

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