[Histonet] blotches in sections

From:"Pool, Madeline"

I'm cutting 10 um sections from paraffin embedded mouse brain, spinal
cord, and optic nerve and staining them with Luxol Fast Blue. In the
brain sections, I'm seeing some splotchy darker background. I'm
wondering if maybe I didn't dry the slides enough after sectioning or
maybe heated it too much or something else entirely. If anyone has seen
things like this before and can tell me what I should do to fix it,
please let me know. I've put a picture of a section up at:
http://members.rogers.com/madeline.pool/brain.jpg (Don't worry about the
tearing, I was testing out bad sections.)

Thanks in advance,

Madeline Pool
Ottawa Health Research Institute
501 Smyth Road
Ottawa, Ontario
K1H 8L6
fax: (613)737-8023

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