[Histonet] Schiffs reagent viability test, qualitative
10 mls formaldehyde, I often use neutral buffered formalin and add a few
drops of Schiffs reagent to this with a pasteur pipette. Use a clear glass
beaker so you can see color development easily. The Schiffs reacts
with the -aldehyde, and fresh, new Schiffs will turn a very bright
dark, red-pink color VERY rapidly.
I suggest you try this with fresh newly opened reagent to see what color
you get - this will help you calibrate your eye to the color that develops.
One caveat: do NOT pour used Schiffs back into the Stock solution
bottle, we store used Schiffs that is contaminated with water carryover
from slides (dilution factor) in a separately labeled bottle and if we
reuse this, do the test first or toss used Schiffs if only using a few mls
i.e. 20 mls or so. Also, if Schiffs develops a precipitate, we toss it and
we never allow it to freeze.
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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