[Histonet] Re: Counterstain for Von Kossa.

From:Jennifer Sipes

I can give you the protocol that I have.

   Deparrafinize and rehydrate to dH2O.
   5% Silver Nitrate solution, place in bright sunlight or in front of a 60 watt light bulb (place foil or mirror behind jar to reflect light.  Leave for one hour or until calcium turns black.
   Rinse in dH2O, 3 changes.(I usually just use running dH2O for about 10-20 minutes)
   5% NaThioSulfate for 5 min.
   Wash in tap H2O, rinse in distilled.
   Nuclear fast red for 5 minutes.
   Wash in water.
   Dehydrate and coverslip.
   Crystals will be black and cellular structures will be red/pink.
If you have any more questions, feel free to write me.

Jennifer K. Sipes, RALAT
Sr. Laboratory Technician
Johns Hopkins University
Ross 929
720 Rutland Avenue
Baltimore, MD  21205
phone:  410-614-0131
cell:     443-413-0853
e-mail:  jengirl1014@yahoo.com

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