Way back in the day, Brigati was using PBS on his immunostainer to blue
after the hematoxylin counterstain.  I have since used PBS to blue my
slides instead of ammonium hydroxide/water.  It's cheap, and very easy
on the sections, and I have plenty of it on hand.
 

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110
tjj@stowers-institute.org


 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet