Fwd: [Histonet] Re: fine red ppt on sections after AP Envision.
>In addition to Joanne's reply, using PBS rinsing at chromogen steps can
>provide phosphate ions that will react with the AP. Either use TRIS
>buffered saline throughout whole staining protocol or at least do a TBS
>rinse x 3 before the AP chromogen step to get rid of phosphate ions.
>Another nice block for endogenous Alk phos is KPL's ready to use universal
>blocker, done at beginning of staining protocol ( just like peroxidase
>blocking, up front). Nice thing is you don't have to add anything to
>the chromogen at the end. This block nicely replaces levamisole.
KPL, you've got me there. Where do I buy this universal blocker.
>At 09:10 AM 11/18/2004, you wrote:
>>Have you tried adding levamisole to your chromogen? It blocks endogenous
>>alk-phos in tissue.Dako sells it seperately from kit. I use 1 drop per
>>ml. of chromogen, and it works quite well.
Dr. Ian Montgomery,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Tel: 0141 339 8855
Pager: 07625 702883
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