Re: [Histonet] antigen for B-cells in bone marrow

From:"Bryan Hewlett"

John,

You are correct!
CD79a exhibits a wider range of B cell staining than CD20, labelling both
earlier and later forms.

Regards,

Bryan

----- Original Message -----
From: "JOHN PHILLIPS" 
To: "Mildred Fail" ; ;

Sent: Wednesday, November 10, 2004 4:47 AM
Subject: RE: [Histonet] antigen for B-cells in bone marrow


I note someone has advocated CD20 staining for Bcell in bone marrow:

I do not profess to know much about these matters but from my limited
experience in performing immuno work on trephine biopsies i think that early
Bcell forms may be missed if CD20, is investigated on its own. CD79 antibody
will label early B cell precursor cells and so should be performed in
conjunction with or perhaps instead of CD20. That is where my knowedge and
understanding fails me.

I have chosen to contact the "Histonetters" who i am sure will sort this out
and as a consequence i will learn from sticking my oar in. That's what this
is all about, ain't it.

John, Wrexham, Wales.

-----Original Message-----
From: Mildred Fail [mailto:failm@musc.edu]
Sent: 09 November 2004 21:47
To: scoop@mail.nih.gov; histonet@pathology.swmed.edu
Subject: Re: [Histonet] antigen for B-cells in bone marrow


CD20- clone L-26,DAKO, 1:2000  HIER with Citrate Buffer. We do it on BMs
almost every day, beautiful every time
Rena Fail


>>> Sharon Cooperman  11/05/04 06:42PM >>>
Dear Histonetters,

Does anyone know of an antibody that works well on B-cells in bone
marrow?  I'm actually trying to use it on a western blot, but I think
many Abs that work on IHC work on western.  I've tried CD11a, CD11b,
and Pax-5, all of which are supposed to work on western but none did
(maybe I need to load more protein).  Any suggestions would be
greatly appreciated.

Thanks,
Sharon
--
Sharon Cooperman             
NIH, NICHD, CBMB                     301.435-8417
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Mae'r e-bost hwn ac unrhyw ffeiliau a drosglwyddir gydag ef yn
gyfrinachol ac wedi'u bwriadu ar gyfer pwy bynnag y cyfeirir ef ato neu
atynt.  Os ydych wedi ei dderbyn drwy gamgymeriad yna gadewch i'r
rheolwr systemau wybod drwy ddefnyddio'r manylion isod.

Mae cynnwys yr e-bost hwn yn cynrychioli barn y sawl a enwir uchod,
felly nid ydyw'n dilyn ei fod yn cynrychioli barn GIG Gogledd Ddwrain
Cymru.

Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i
Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru roi cyhoeddusrwydd i gynnwys
unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodauâ?Tr Ddeddf
Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth,
cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru
ar  www.newalesnhstrust.org.uk


This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed.  If you have received this email in error please notify
the system manager using the details below.

The contents of this email represent the views of the individual(s)
named above and do not necessarily represent the views of the
North East Wales NHS Trust.

Please be aware that, under the terms of the Freedom of Information Act
2000, the North East Wales NHS Trust may be required to make public the
content of any emails or correspondence received.  For futher
information on Freedom of Information, please refer to the North East
Wales NHS Trust website at www.newalesnhstrust.org.uk

For further assistance, please contact
system.administrator@new-tr.wales.nhs.uk.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>