Re: [Histonet] a much easier chemistry question

From:Ford Royer

Years ago, Scientific Products made a lanolin-based cream stain remover 
that worked great in removing biological stains.  It worked like magic 
on stain on hands and lab surfaces.  One day, out of frustration, I 
tried it on my white lab coat (stained with methylene blue) and it 
worked fine.  I rubbed the cream into the stain, and then washed to coat 
in the washing machine... stain all gone!  I check a recent (1997) 
Allegiance catalog, and they still showed this product.  Called 
"Dye-Sol" and the catalog number was C6345.  All the S/P products are 
now carried by Cardinal so you could contact them to see if they still 
carry it.  They should be able to cross-reference the old Allegiance 
cat. no.

Good Luck!

~ Ford

Ford M. Royer, MT(ASCP)
Midwest Science Biocenter
Minneapolis, MN

John Kiernan wrote:

>Your unsuccessful efforts were all acidic, 
>neutral or hydrophobic. Trypan and Evans blue are 
>anionic dyes with large, very hydrophilic molecules.
>Alkaline solutions extract anionic dyes, and urea 
>(high concentration) can disrupt the non-ionic, 
>non-covalent bonding that holds large hydrophilic
>dye molecules to substrates such as cellulose (cotton). 
>Try a strong urea solution (8M=48%) made alkaline
>with borax or washing soda. Geoff McAuliffe's
>simpler suggestion of soap and water may work,
>because soap is alkaline. 
>Keep us informed. All who use dyes need this sort
>of information. (I have a grey tie with alcian 
>blue spots dating from the 1960s; wouldn't want to 
>remove them now, though I'm sure it would be
>impossible without dissolving the fabric.)
>John Kiernan
>London, Canada
>Jack England wrote:
>>Aloha all,
>>Here's a much more fun chemistry question for anyone that wants to bite:
>>what is the best method to remove trypan/evans blue from cotton fabric?  One
>>of my colleagues got some on her pants the other day and asked what the best
>>way to get it out was.  I did not know, so I put some on cotton gauze, and
>>--acid alcohol...faded the stain a little, but didn't get rid of it
>>--DI change
>>--absolute change
>> change
>>--ethanol/non-ionic detergent/DI water change
>>--citrus oil change
>>Given that her fabric is tan (and thus not white, and thus likely not
>>bleach-able), can anyone out there in histo-land suggest a way of
>>de-staining trypan-blue-stained fabric? I've been wondering about this for a
>>while now and figured I'd pass it around.
>>--Many thanks and aloha to all,
>>Jack England
>>Tissue Genesis, Inc.
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